That will be up to you as you're the one performing the service.
** If you think it is still not clear due to scam **
That would be scum.
* kindly advise on how to clean the tubing? **
There is no set procedure. Some technicians use compressed air from one end or the other, some use pressurized water while other insert a flexible implement down it and twist it like a rotor router then flush it with warm water under some pressure like can sometimes be achieved using a turkey baster.
** What about if the thermostat switches into cooling mode too early and therfore skipping the delay time? **
You can read how a frost-free refrigerator's defrost system functions at the following link:
** Now testing it at 1... normally I ran the fridge at setting 3 which is normal **
If the thermostat is no longer controlling the temperature adequately or as it used to, it may be loosing its calibration with age and might need to eventually be replaced.